Different output properties of perisomatic region-targeting interneurons in the basal amygdala

Barsy, B and Szabó, G.M. and Andrási, Tibor and Vikór, A. and Hájos, Norbert (2017) Different output properties of perisomatic region-targeting interneurons in the basal amygdala. EJN. (In Press)


Download (19MB) | Preview


Perisomatic region of principal neurons in the cortical regions is innervated by three types of GABAergic interneurons, including parvalbumi n - containing basket cells (PVBCs) and axo - axonic cells (AACs), as well as cholecystokinin and type 1 cannabinoid receptor - expressing basket cells (CCK/CB1BCs). These perisomatic inhibitory cell types can also be found in the basal nucleus of the amygdala, however, their output properties are largely unknown. Here, we performed whole - cell recordings in morphologically identified interneurons in slices prepared from transgenic mice, in which the GABAergic cells could be selectively targeted. By investigating the passive and active membrane properties of interneurons located within the bas al amygdala , w e observed that the three interneuron types had distinct single - cell properties . For instance, the input resistance, spike rate, accommodation in discharge rate , or after - hyperpolarization width at the half maximal amplitude separated the three interneuron types . Furthermore , we performed paired recordings from interneurons and principal neurons to uncover the basic features of unitary inhibitory postsynaptic curr ents (uIPSCs). We found that, although there was no difference in the magnitude of responses measured in the principal neurons , the uIPSCs originated from the distinct interneuron types differed in the rise time, failure rate, latency and short - term dynami cs. Moreover, the asynchronous transmitter release induced by a train of action potentials was typical for the output synapses of CCK/CB1BCs. Our results suggest that , although the three perisomatic inhibitory cell types give rise to uIPSCs with similar ma gnitude, their distinct spiking characteristics may help to accomplish specific function in amygdala operation.

Item Type: Article
Subjects: Q Science / természettudomány > QP Physiology / élettan
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: Dr. Boglárka Barsy
Date Deposited: 22 Jan 2017 20:31
Last Modified: 04 Apr 2023 12:07

Actions (login required)

Edit Item Edit Item