New type biosensor for the detection of pesticides, based on the inhibition of acetylcholinesterase

Csiffáry, G. and Nagy, P. and Kiss, A. and Adányi, N. (2013) New type biosensor for the detection of pesticides, based on the inhibition of acetylcholinesterase. Acta Alimentaria, 42 (1). pp. 79-90. ISSN 0139-3006

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Abstract

An amperometric biosensor for the detection of organophosphorus and carbamate type pesticides was developed. Acetylcholinesterase enzyme (electric eel) was immobilized in a thin-layer enzyme cell, and acetylthiocholine chloride (AcTCh) was used as substrate. Amperometric detection with glassy carbon electrode was performed in a stopped-flow FIA system. The inhibition effect of dichlorvos (DDVP), carbofuran, methomyl, and pirimicarb were examined: the linear measuring ranges were 0.001–0.1 μmol l−1, 0.001–0.1 μmol l−1, 0.2-1 μmol l−1, and 0.1–10 μmol l−1, respectively. Soil extract and apple juice were measured with spiking method. It was concluded that the biosensor can be used for screening pesticide residues in food and environmental samples.

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