Detection of a metabolite produced by acidophilic spoilage-causing bacteria using different analytical and sensory methods

Farkas, V. and Dalmadi, I. (2013) Detection of a metabolite produced by acidophilic spoilage-causing bacteria using different analytical and sensory methods. Acta Alimentaria, 42 (Supple). pp. 19-26. ISSN 0139-3006

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Abstract

In this study, detection of an obligate aerobic, thermophilic and acidophilic bacterium, the sporeforming Alicyclobacillus acidoterrestris was performed by determination of its specifc metabolite, guaiacol. Since its spores have been shown to resist conventional pasteurization, it has become a potential spoilage concern for fruit and vegetable juices, mainly for apple and orange juices. Detection of guaiacol was carried out by using an NST 3320 type electronic nose, and other methods, such as peroxidase-based enzymatic method with UV-Vis spectrophotometer, SPME-GC-MS technique and an untrained sensory panel were also applied. The results indicated that based on their detection limit the methods can be ordered in the following way: SPME-GC-MS (detection limit: <0.5 ppm)<sensory evaluation (detection limit: 0.5–1 ppm)<spectrophotometric method=electronic nose technique (detection limit: 1.25–2.5 ppm).

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