Adsorption of ochratoxin a by Saccharomyces cerevisiae living and non-living cells

Piotrowska, M. (2012) Adsorption of ochratoxin a by Saccharomyces cerevisiae living and non-living cells. Acta Alimentaria, 41 (1). pp. 1-7. ISSN 0139-3006

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Abstract

In presented work the ability of Saccharomyces cerevisiae cells to binding of ochratoxin A was evaluated. The viable and thermally inactivated cells at various densities were incubated with ochratoxin A in PBS buffer and the toxin residue in supernatant was determined using HPLC method. The amount of OTA removed after 24 h of contact equalled 20, 29 and 75% for 1, 5 and 50 mg of dry biomass/ml, respectively. It was proved that killed biomass remove OTA from buffer in higher quantities than viable cells. The process of adsorption proved to be very fast; 30 min after mixing the cells with toxin, its amount significantly decreased. It was stated that incubation of the samples under static conditions is more effective than incubation with shaking. The releasing from 11 to 22% of initially binding toxin after three washing of biomass indicates the reversibility of bond between cells and toxins. The complex of killed cells-ochratoxin A is less firm than it is in case of viable biomass. Adsorption of toxin is closely related to the components of yeast’s cell wall. Cells without cell wall (protoplasts) lost the ability to adsorption of ochratoxin A.

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