Direct HPLC-UV determination of cyclamate, saccharine and aspartame from soft drinks

Croitoru, M. and Fülöp, I. and Ajtay, M. and Balogh, C. and Dogaru, M. (2011) Direct HPLC-UV determination of cyclamate, saccharine and aspartame from soft drinks. Acta Alimentaria, 40 (4). pp. 459-465. ISSN 0139-3006

Text aalim.40.2011.4.5.pdf Restricted to Repository staff only until 31 December 2031. Download (367kB)

Abstract

Artificial sweeteners were introduced in therapy as sugar substitutes for diabetic patients. Nowadays these substances are widely used for sugar substitution in low calorie drinks and sweets. Most commonly used products to date are saccharine, cyclamate, aspartame, and acesulfam; maximum accepted daily intakes are stated for each one.A simple reverse phase (RP18) HPLC-UV method with direct detection (196 nm) was developed by us in order to measure the concentrations of the three sweeteners. No sample preparation is required, other than dilution. Limits of detection are 12 mg l−1, 0.5 mg l⁻¹ and lower than 0.25 mg l−1 for cyclamate, aspartame and saccharine, respectively. Concentrations ranged between 113.14–280.07 mg l−1 in the case of cyclamate, 17.96–50.94 mg l-1 for saccharine, and 9.94–296.82 mg l−1 for aspartame.

Actions (login required)

Edit Item