Induction of pathogenesis-related proteins in intercellular fluid by cadmium stress in barley (Hordeum vulgare L.) — A proteomic analysis

Pós, V. and Hunyadi-Gulyás, É. and Caiazzo, R. and Jócsák, I. and Medzihradszky, K. and Lukács, N. (2011) Induction of pathogenesis-related proteins in intercellular fluid by cadmium stress in barley (Hordeum vulgare L.) — A proteomic analysis. Acta Alimentaria, 40 (Supple). pp. 164-175. ISSN 0139-3006

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Abstract

The effect of cadmium on protein expression in the aerial parts of barley (Hordeum vulgare L. cv. ‘Mandolina’) seedlings was investigated by proteomic analysis of leaf apoplast proteins. Dramatic changes were observed in the protein pattern of intercellular washing fluid from Cd-treated (0–300 μM) barley leaves both by 1D- and 2D-PAGE. By mass spectrometric (MALDI-TOF and/or LC-MS/MS) analysis of induced proteins PR1 proteins, certain 1-3-glucanases (PR2), chitinases (PR3), members of the chitin binding PR4 family, a rich set of thaumatin-like proteins (PR5) and two PR17 proteins were identified, indicating that a general plant defence response, inducing massive secretion of pathogenesis-related proteins (PR) into the extracellular space, is an important part of the Cd-induced stress reactions. Although systemic induction of PR proteins is probably important for an adequate plant response to cadmium stress, many of these proteins are known to have an allergenic potential and as such present a health risk to plant eaters, even when the heavy metal concentration in the given plant organ is low.

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