Characterization of 2-propanol soluble seed proteins in mutant soybean ( Glycine max [L.] Merr.) lines

Novák-Hajós, M. and Szamos, J. and Gasztonyi, M. and Hajós, Gy. (2010) Characterization of 2-propanol soluble seed proteins in mutant soybean ( Glycine max [L.] Merr.) lines. Acta Alimentaria, 39 (2). pp. 175-179. ISSN 0139-3006

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Abstract

Seed protein pattern of control and M10 mutant soybean (Glycine max [L.] Merr.) lines in defatted and non-defatted raw flour was studied after 60% 2-propanol extraction, SDS-PAGE separation, colloidal staining and densitometric evaluation to detect a new variant of the protein KTI and/or BBI, furthermore to find new protein(s) of low molecular weight. Electrophoretic separation of defatted and non-defatted control soybean samples showed the same protein patterns. On the densitograms of mutant lines quantitative and qualitative differences could be observed. Defatted raw soy samples reflected more differences in the number of peaks than non-defatted ones. Beside soy trypsin inhibitors, several more soy proteins of low molecular weight are dissolved. KA mutant line 9 has a unique 2-propanol soluble protein pattern, and a new protein band of Rf=0.37 compared to the control line. Sixty percent 2-propanol soluble soybean seed proteins are suitable for cultivar identification and characterization, furthermore to distinguish soybean lines of the same origin.

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