An occurrence of genetically modified foodstuffs on the Czech food market

Kyrova, V. and Ostry, V. and Laichmannova, L. and Ruprich, J. (2010) An occurrence of genetically modified foodstuffs on the Czech food market. Acta Alimentaria, 39 (4). pp. 387-396. ISSN 0139-3006

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Abstract

Genetically modified (GM) plants are obtained by adding to them one or more foreign genes that encode new properties, such as tolerance to herbicides, resistance to insects and the ability to produce new substances. The aim of this study was the detection and identification of GM foodstuffs. Six different types of samples (soybeans, soya products, tomatoes, maize flour, rice and papaya) were collected at 12 places in the Czech Republic during the years 2002–2007. It represents a total of 1225 samples of foodstuffs.Samples were investigated for the presence of material derived from the following genetically modified organisms (GMOs) which are approved for food use in the European Union (EU): Roundup Ready soybean (RRS) and maize lines Bt176, Bt11, T25, GA21, MON810, DAS1507 and some non-approved in the EU: maize lines Bt10 and starlink, rice, tomatoes and papaya. Polymerase chain reaction (PCR)-based methods and enzyme-linked immunosorbent assay (ELISA) were used for the detection of GM foods.RRS was detected in 14 (4.9%) samples of soybean out of 288 and in 88 (30.5%) soya products out of 288 samples. The amount of RRS in positive samples was determined by quantitative PCR. The content was in the range of 0.01–75.3% RRS. GM maize was detected in 5 (1.7%) of 288 samples. Maize lines MON810, Bt176 and StarLink were detected in the maize samples. GM rice was detected in 2 (1.9%) samples out of 102. All investigated tomatoes and papaya samples were negative for detection of GM.

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