PCR-Based assays for the identification of enniatin-producing Fusarium species associated to wheat

Koncz, Zs. and Naár, Z. and Kiss, A. and Szécsi, Á. (2009) PCR-Based assays for the identification of enniatin-producing Fusarium species associated to wheat. Acta Alimentaria, 38 (4). pp. 483-492. ISSN 0139-3006

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Abstract

Enniatins (ENs), produced by Fusarium species are a group of mycotoxins with antimicrobial, insecticidal (GROVE & POPLE, 1980) and phytotoxic activities. PCR based assays were applied for detecting enniatin-producing strains of Fusarium avenaceum, F. poae and F. sporotrichioides isolated from wheat seeds originated of 30 geographic localities of Hungary. All F. sporotrichoides strains and except two of all F. poae strains gave positive signal to esysp1 and esysp2 primers as well as all F. avenaceum isolates were positive to esya1 and esya2 primers indicating the ability to produce ENs. This is a first report of the enniatin producing ability of Fusarium species associated to wheat in Hungary.

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