Determination of folic acid in enriched dairy products

Catharino, R. and Lima, J. and Godoy, H. (2007) Determination of folic acid in enriched dairy products. Acta Alimentaria, 36 (1). pp. 139-147. ISSN 0139-3006

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Abstract

In Brazil, dairy products are being enriched with folic acid (FA), an important vitamin for public health. With a view to guaranteeing a degree of confidence in these enriched products, the objective of this research was to determine the levels of FA in enriched dairy products such as powdered milk, sterilised milk, dairy beverages and ‘petit suisse’ cheese. The methodology used to determine FA by high performance liquid chromatography (HPLC) used a 0.1 mol 1 ⁻¹ KOH solution for extraction and trichloracetic acid for the clean up. A C ₁₈ column was used for the separation with gradient elution using a mobile phase of acetate buffer and acetonitrile, which allowed for the completion of the analysis in 9 min. Detection was carried out at 290 nm and quantification by the way of an external standard. Of the twenty-five products analysed, sixteen presented FA values with only slight deviations from the values declared, whereas seven showed very low values, mainly sterilised milks and ready to drink dairy beverages, and in two of the three ‘petit suisse’ cheeses no vitamin was detected.

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