Nutritional evaluation of the proteins of broad range herbicide resistant spring wheat lines (Triticum aestivum L.) I. Protein quality

Nagy, A. and Baráth, Á. and Pauk, J. and Gelencsér, É. (2006) Nutritional evaluation of the proteins of broad range herbicide resistant spring wheat lines (Triticum aestivum L.) I. Protein quality. Acta Alimentaria, 35 (3). pp. 355-362. ISSN 0139-3006

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Abstract

The authors have developed transgenic wheat lines with broad range of herbicide resistance. The transgenic wheat, containing bacterial derived alien gene (bar) regulated under the maize ubiquitin promoter, is resistant to glyphosinate (phosphinotrichin) agent family. The presence of bar gene expression product (phosphinotrichin acetyl transpherase enzyme, PAT) was confirmed by PAT-specific ELISA (Enzyme Linked Immuno Sorbent Assay). The qualitative and quantitative chemical composition of the transgenic wheat lines in comparison with their non-transgenic counterpart (year 2000-2002) and protein utilization of the wheat wholemeal flours (year 2002) were summarized. Nutritional evaluation of the protein was based on a rat model by using N-balance experiments. Among the protein sources, heat-treated samples were also introduced into the experimental diets. It was found that heat denaturation of the proteins led to results with somewhat increased biological value indices. The introduction of GM technology did not affect food intake or nutritional performance of the rats.

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