Jánosi, A. and Szamos, J. (2001) Comparison of two methods in purification of meat-DNA for PCR. Acta Alimentaria, 30 (1). pp. 113-118. ISSN 0139-3006
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Abstract
The Wizard Clean up System and a three-phase partitioning (TPP) method were used to purify genomic pork-DNA of various food samples for amplification. Quality of DNA purified by Wizard resin and partitioning was controlled by spectrophotometer and electrophoresis, respectively. A 108 bp fragment from the porcine growth hormone gene was applied, according to M EYER and co-workers (1994). Of all the samples prepared, amplicons were obtained by the pork- DNA specific PCR. Partitioning was found to be an efficient DNA purification step in preparation of PCR-grade DNA.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
| Depositing User: | xKatalin xBarta |
| Date Deposited: | 17 Feb 2017 09:36 |
| Last Modified: | 28 Feb 2021 00:15 |
| URI: | http://real.mtak.hu/id/eprint/49336 |
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