Factors affecting cellulase production by trichoderma koningii

El-hawary, F. I. and Mostafa, Y. S. (2001) Factors affecting cellulase production by trichoderma koningii. Acta Alimentaria, 30 (1). pp. 3-13. ISSN 0139-3006

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Abstract

Because of the need for renewable energy resources, cellulose, which can be enzymatically hydrolyzed to glucose, has drawn lot of attention during the past decade. However, the process of cellulose conversion using cellulase is not yet economically feasible because of the high cost of enzymes. Factors influencing the cellulase production of Trichoderma koningii using both acid and steam treated sugar cane bagasse and rice straw as carbon sources were investigated. The highest levels of cellulase activities were obtained using a culture medium containing urea and (NH4)2SO4 together as nitrogen sources at 0.217% and 0.241% for both carbon sources. When the culture medium was supplemented either with 0.5% Tween 60 or Tween 80, the rate of cellulase production was increased considerably. Maximum levels of both filter paper and CMC-ase activities produced on both media were obtained at 25 °C and 100 r.p.m., while the highest level of â -glucosidase production was obtained at 30 °C and 200 r.p.m.

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