Preliminary communications. Cellulase production and conversion of rice straw to lactic acid by simultaneous saccharification and fermentation

Mostafa, Y. S. and László, E. and El-hawary, F. I. (2001) Preliminary communications. Cellulase production and conversion of rice straw to lactic acid by simultaneous saccharification and fermentation. Acta Alimentaria, 30 (3). pp. 281-295. ISSN 0139-3006

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Abstract

Factors affecting the cellulase production of Aspergillus niger using sugar cane bagasse as carbon source were investigated. The highest enzyme activities were obtained, when the culture medium was supplemented with 0.133% tryptone as nitrogen source. The rate of cellulase production was considerably increased when 0.5% Tween 60 was added to the production medium. For FPA and b-glucosidase production pH 5.0, while for CMC-ase pH 5.5 was found to be optimal. The highest cellulase activities were obtained at 30 °C and 300 r.p.m. The highest saccharification degree was achieved, when alkali treated rice straw was used as substrate. The main objective of the present study was to examine the possibilities of lactic acid production from alkali treated rice straw using simultaneous saccharification and fermentation technique with T. koningii cellulases and L. delbrueckii. The highest conversion of cellulose was obtained using 6% alkaline treated rice straw supplemented with 1.2 mg enzyme/g substrate at pH 4.8 and 45 °C.

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