Pyridoxine analysis by high performance liquid chromatography and validation in fortified milk powder

Amaro, C.O. and Flores, C. and Dias, M.G. and Lidon, F.C. (2014) Pyridoxine analysis by high performance liquid chromatography and validation in fortified milk powder. Acta Alimentaria, 43 (2). pp. 297-305. ISSN 0139-3006

Preview

Text aalim.43.2014.2.14.pdf Download (445kB) | Preview

Abstract

Vitamin B₆ analysis by reverse-phase high performance liquid chromatography and its validation in milk powder prompted this study. The calibration curves for pyridoxal, pyridoxamine and pyridoxine were evaluated and method precision and accuracy assessed. It was found that the working range was adequate for all the analytes. The analytical procedure was verified by testing the enzymatic efficiency, precision and accuracy. In milk powder samples pyridoxine was the only vitamer found in quantities above the considered limit of quantification and the accuracy showed Z-scores lower than 2. Uncertainty estimation based on method validation results was 0.066 mg/100g, for a vitamin B₆ content of 0.61 mg/100 g. It was concluded that the method is adequate for vitamin B₆ quantification in powder milk.

Actions (login required)

Edit Item