Tölgyesi, Á. and Fekete, J. and Sharma, V.K. and Pálffi, É. and Békési, K. and Lukonics, D. and Pleva, G. (2014) A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay. Acta Alimentaria, 43 (2). pp. 306-314. ISSN 0139-3006
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Abstract
A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 μg kg<sup>−1</sup>. All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 μg kg<sup>−1</sup> for CAP in each validated matrix, while the detection capability of the screening test was 0.15 μg kg<sup>−1</sup>.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
| Depositing User: | Erika Bilicsi |
| Date Deposited: | 04 Mar 2017 11:54 |
| Last Modified: | 04 Apr 2023 12:34 |
| URI: | http://real.mtak.hu/id/eprint/49850 |
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