Survivability of lactobacilli cells upon coating with methacrylic acid copolymers

Tham, C.S.C. and Peh, K.K. and Liong, M.T. (2014) Survivability of lactobacilli cells upon coating with methacrylic acid copolymers. Acta Alimentaria, 43 (2). pp. 218-224. ISSN 0139-3006

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Abstract

This study aimed to evaluate the coating effect of methacrylic acid copolymers (MAc) on alginate beads, with respect to protection, survivability, and in vitro release of lactobacilli cells under simulated gut conditions, and to evaluate the effect of oven drying and fluidized bed-spraying as a mean to dry and coat alginate beads with MAc. MAc-coated beads exhibited protective effects for lactobacilli cells against low acidic environment providing higher survivability (71.82–96.11%) compared to uncoated beads (28.67–61.68%), and also higher cell release under sequential gut conditions (P<0.05). Lactobacillus casei BT 1268 showed a high cell release and was thus selected for further analysis using spray coating-drying via fluidized bed. Scanning electron microscopy revealed that fluidized bed-spray drying produced beads with irregular shapes but consistent coating and with lactobacilli cells clearly embedded within the inner matrix. Oven dried and fluidized bed-spray dried MAc-coated beads of L. casei BT 1268 also showed similar cell release, indicating the possible use of fluidized bed-spray as a more economical and less time consuming method for the protection and maintenance of cell viability.

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