In-situ Clean-up and OPLC Fractionation of Chamomile Flower Extract Searching Active Components by Bioautography

Mincsovics, Emil and Ott, Péter G. and Alberti, Ágnes and Böszörményi, Andrea and Héthelyi, Éva B. and Szőke, Éva and Kéry, Ágnes and Lemberkovics, Éva and Móricz, Ágnes M. (2013) In-situ Clean-up and OPLC Fractionation of Chamomile Flower Extract Searching Active Components by Bioautography. Journal of Planar Chromatography - Modern TLC, 26 (2). pp. 172-179. ISSN 0933-4173

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Abstract

Bioassay-guided isolation of antibacterial components of chamomile flower methanol extract was performed by OPLC with on-line detection, fractionation combined with sample clean-up in-situ in the adsorbent bed after sample application. The antibacterial effect of the fractions and the separated compounds remained on the adsorbent layer (do not overrun during OPLC separation) was tested with direct bioautography (DB) against the bioluminescent Pseudomonas savastanoi pv. maculicola and Vibrio fischeri. The fractions with great biologically activity were analysed by SPME-GC-MS and LC-MS/MS and the two active uneluted compounds were characterized by OPLC-MS using interface. Mainly essential oil components, coumarins, flavonoids, phenolic acids and fatty acids were identified in the fractions.

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