Móricz, Ágnes M. and Ott, Péter G. and Alberti, Ágnes and Böszörményi, Andrea and Lemberkovics, Éva and Szőke, Éva and Kéry, Ágnes and Mincsovics, Emil (2013) Applicability of Preparative Overpressured Layer Chromatography and Direct Bioautography in Search of Antibacterial Chamomile Compounds. Journal of AOAC International, 96 (6). pp. 1214-1221. ISSN 1060-3271, ESSN: 1944-7922
![]() |
Text
SGE_Moricz.pdf - Published Version Restricted to Repository staff only Download (1MB) | Request a copy |
Abstract
In situ sample preparation and preparative overpressured layer chromatography (OPLC) fractionation on a 0.5 mm thick adsorbent layer of chamomile flower methanol extract prepurified by conventional gravitation accelerated column chromatography were applied in searching for bioactive components. Sample cleanup in situ on the adsorbent layer subsequent to sample application was performed using mobile phase flow in the opposite direction (the input and output of the eluent was exchanged). The antibacterial effect of the fractions obtained from the stepwise gradient OPLC separation with the flow in the normal direction was evaluated by direct bioautography against two Gram-negative bacteria: the luminescence gene tagged plant pathogenic Pseudomonas syringae pv. maculicola, and the naturally luminescent marine bacterium Vibrio fischeri. The fractions having strong activity were analyzed by SPME-GC/MS and HPLC/MS/MS. Mainly essential oil components, coumarins, flavonoids, phenolic acids, and fatty acids were tentatively identified in the fractions.
Item Type: | Article |
---|---|
Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
Depositing User: | Dr. Ágnes Móricz |
Date Deposited: | 17 Jan 2014 10:41 |
Last Modified: | 24 Mar 2014 11:44 |
URI: | http://real.mtak.hu/id/eprint/8956 |
Actions (login required)
![]() |
Edit Item |