Ács, András and Ozohanics, Oliver and Vékey, Károly and Drahos, László and Turiák, Lilla (2018) Distinguishing core and antenna fucosylated glycopeptides based on low energy tandem mass spectra. ANALYTICAL CHEMISTRY, 90 (21). pp. 12776-12782. ISSN 0003-2700
|
Text
Manuscript File_Anal_Chem.pdf Download (746kB) | Preview |
Abstract
A straightforward approach has been developed to distinguish core and antenna fucosylation in glycopeptides. The method does not require derivatization, and can be easily adapted into a proteomics workflow. The key aspect is to use low collision energy CID (on a QTOF type instrument) when only single step fragmentation processes occur. Low collision energy should show the precursor ion as the largest peak in the spectrum; the survival yield should be ideally over 50%; and this is obtained at a collision energy ca. 30% of that typically used for proteomics. In such a case interfering processes like fucose migration or consecutive reactions are minimized. Core and antenna fucosylation can be discriminated using various ion abundance ratios. Low energy CID spectra are very “clean” (no chemical noise), and the ions used for locating the fucose are among the major peaks; making the method well suited for analytical work. Monitoring the change in the proportion of core and antenna fucosylation at the same glycosylation site is also feasible.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | mass spectrometry, glycopeptide, fragmentation, CID, energy-dependent, fucosylation |
Subjects: | Q Science / természettudomány > Q1 Science (General) / természettudomány általában Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
Depositing User: | Ágnes Gömöry |
Date Deposited: | 31 Jan 2019 09:33 |
Last Modified: | 05 Apr 2023 07:58 |
URI: | http://real.mtak.hu/id/eprint/90874 |
Actions (login required)
![]() |
Edit Item |