Reproducibility analysis of bioimpedance-based self-developed live cell assays

Vizvári, Zoltán Ákos and Győrfi, Nina Rubina and Maczko, Gergo and Varga, Reka and Jakabfi-Csepregi, Rita and Sári, Zoltán and Füredi, András and Bajtai, Eszter and Vajda, Flóra and Tadity, Vladimir and Odry, Péter and Karádi, Zoltán György and Tóth, Attila (2024) Reproducibility analysis of bioimpedance-based self-developed live cell assays. SCIENTIFIC REPORTS, 14 (1). No. 16380. ISSN 2045-2322

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Abstract

Bioimpedance spectrum (BIS) measurements have a great future in in vitro experiments, meeting all the requirements for non-destructive and label-free methods. Nevertheless, a real basic research can provide the necessary milestones to achieve the success of the method. In this paper a self-developed technology-based approach for in vitro assays is proposed. Authors invented a special graphene-based measuring plate in order to assess the high sensitivity and reproducibility of introduced technique. The design of the self-produced BIS plates maximizes the detection capacity of qualitative changes in cell culture and it is robust against physical efects and artifacts. The plates do not infuence the viability and proliferation, however the results are robust, stable and reproducible regardless of when and where the experiments are carried out. In this study, physiological saline concentrations, two cancer and stem cell lines were utilized. All the results were statistically tested and confrmed. The fndings of the assays show, that the introduced BIS technology is appropriate to be used in vitro experiments with high efcacy. The experimental results demonstrate high correlation values across the replicates, and the model parameters suggested that the characteristic diferences among the various cell lines can be detected using appropriate hypothesis tests.

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