Poór, Miklós and Gömbös, Patrik and Szabó, András and Zsidó, Balázs Zoltán and Hetényi, Csaba and Huber, Tamás and Lukács, András Szilárd and Kunsági-Máté, Sándor (2025) Interaction of Mycotoxins with α1-Acid Glycoprotein (AGP) and Bovine Milk Proteins: Zearalenone, Zearalenols, and Sterigmatocystin Form Highly Stable Complexes with AGP. TOXINS, 17 (4). No.-151. ISSN 2072-6651
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Abstract
Mycotoxins are frequent food contaminants posing health risk to humans and animals. Since these interactions have been barely studied yet, we examined the potential complex formation of mycotoxins with human α1-acid glycoprotein (AGP) and with bovine milk proteins (including casein (CSN), β-lactoglobulin (LG), and α-lactalbumin (LA)) based on fluorescence spectroscopic and ultracentrifugation techniques. Only weak interactions (logK = 2.7 to 3.5) of certain mycotoxins were observed with CSN, LG, and/or LA. Ultracentrifugation experiments demonstrated that aflatoxin M1, zearalenone, and α-zearalenol form more stable complexes with CSN than with LG or LA. These mycotoxins bound to bovine serum albumin with more than a tenfold higher affinity compared to CSN; nevertheless, it has likely limited importance due to the relatively low levels of BSA in bovine milk. Zearalenone, zearalenols, and sterigmatocystin showed strong interactions with AGP (logK = 5.5 to 6.4), suggesting that AGP may play an important role in the plasma protein binding of these mycotoxins.
| Item Type: | Article |
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| Additional Information: | Department of Laboratory Medicine, Medical School, University of Pécs, Ifjúság útja 13, Pécs, H-7624, Hungary Molecular Medicine Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, H-7624, Hungary Institute of Physiology and Nutrition, Department of Physiology and Animal Health, Agribiotechnology and Precision Breeding for Food Security National Laboratory, Hungarian University of Agriculture and Life Sciences, Gödöllő, H-2103, Hungary HUN-REN-MATE Mycotoxins in the Food Chain Research Group, Hungarian University of Agriculture and Life Sciences, Guba Sándor u. 40, Kaposvár, H-7400, Hungary Pharmacoinformatics Unit, Department of Pharmacology and Pharmacotherapy, Medical School, University of Pécs, Szigeti út 12, Pécs, H-7624, Hungary National Laboratory for Drug Research and Development, Budapest, H-1117, Hungary Department of Biophysics, Medical School, University of Pécs, Szigeti út 12, Pécs, H-7624, Hungary Department of Organic and Medicinal Chemistry, Faculty of Pharmacy, University of Pécs, Honvéd u. 1, Pécs, H-7624, Hungary Green Chemistry Research Group, János Szentágothai Research Centre, University of Pécs, Ifjúság útja 20, Pécs, H-7624, Hungary Export Date: 19 May 2025; Correspondence Address: M. Poór; Department of Laboratory Medicine, Medical School, University of Pécs, Pécs, Ifjúság útja 13, H-7624, Hungary; email: poor.miklos@pte.hu |
| Uncontrolled Keywords: | CASEIN; MYCOTOXINS; ZEARALENONE; Aflatoxins; sterigmatocystin; α1-Acid glycoprotein; Zearalenols; β-lactoglobulin; α-Lactalbumin; Bovine albumin serum; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia R Medicine / orvostudomány > RM Therapeutics. Pharmacology / terápia, gyógyszertan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 02 Sep 2025 09:56 |
| Last Modified: | 02 Sep 2025 09:56 |
| URI: | https://real.mtak.hu/id/eprint/223202 |
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