Pantl, Orsolya and Chiovini, Balázs and Szalay, Gergely and Turczel, Gábor and Kovács, Ervin and Mucsi, Zoltán and Rózsa J., Balázs and Cseri, Levente (2024) Seeing and Cleaving: Turn-Off Fluorophore Uncaging and Its Application in Hydrogel Photopatterning and Traceable Neurotransmitter Photocages. ACS APPLIED MATERIALS & INTERFACES, 16 (41). pp. 55107-55117. ISSN 1944-8244
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Abstract
The advancements in targeted drug release and experimental neuroscience have amplified the scientific interest in photolabile protecting groups (PPGs) and photouncaging. The growing need for the detection of uncaging events has led to the development of reporters with fluorescence turn-on upon uncaging. In contrast, fluorescent tags with turn-off properties have been drastically underexplored, although there are applications where they would be sought after. In this work, a rhodamine-based fluorescent tag is developed with signal turn-off following photouncaging. One-photon photolysis experiments reveal a ready loss of red fluorescence signal upon UV (365 nm) irradiation, while no significant change is observed in control experiments in the absence of PPG or with irradiation around the absorption maximum of the fluorophore (595 nm). The two-photon photolysis of the turn-off fluorescent tag is explored in hydrogel photolithography experiments. The hydrogel-bound tag enables the power-, dwell time-, and wavelength-dependent construction of intricate patterns and gradients. Finally, a prominent caged neurotransmitter (MNI-Glu) is modified with the fluorescent tag, resulting in the glutamate precursor named as GlutaTrace with fluorescence traceability and turn-off upon photouncaging. GlutaTrace is successfully applied for the visualization of glutamate precursor distribution following capillary microinjection and for the selective excitation of neurons in a mouse brain model. © 2024 The Authors. Published by American Chemical Society.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Magyar Tudom?nyos Akad?mia [2020-1.1.5-GYORSITOSAV-2021-00004, 2020-2.1.1, ED-2022-00208]; Ministry of Innovation and Technology of Hungary from the National Research, Development, and Innovation Office [BO/799/21/7, BO/365/23/7, UNKP-23-ME4, UNKP-23-5-BME-460]; Hungarian Academy of Sciences; National Research, Development and Innovation Fund [NKFIH/143650] Funding text: The research was supported by the 2020-1.1.5-GYORSITOSAV-2021-00004 Grant. Project 2020-2.1.1.-ED-2022-00208 has been implemented by the Ministry of Innovation and Technology of Hungary from the National Research, Development, and Innovation Office. Z.M. and L.C. are grateful for the Bolyai Janos Research Scholarships (BO/799/21/7, BO/365/23/7, UNKP-23-ME4, and UNKP-23-5-BME-460) provided by Hungarian Academy of Sciences and the National Research, Development and Innovation Fund. G.S. is grateful for the PD scholarship (NKFIH/143650). Dr. Dora Bogdan, Ms. Eszter Kalydi, and Dr. Csaba Varady are acknowledged for their generous help with NMR and MS analyses. The authors are grateful to Dr. Andrius Plauska for his work on the image conversion script used for photolithography. The authors thank Ms. Anett Matuscsak and Mr. Miklos Madarasz, Mr. Attila Csomos, and Dr. Hai Anh Le Phuong for their assistance with the cytotoxicity assay, the two-photon cross section measurements, and the proofreading, respectively. |
| Uncontrolled Keywords: | Cage compounds, Fluorescence, Neurotransmitters, Photolysis, Photolithography |
| Subjects: | Q Science / természettudomány > QC Physics / fizika > QC173.4 Material science / anyagtudomány |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 24 Sep 2025 14:24 |
| Last Modified: | 24 Sep 2025 14:24 |
| URI: | https://real.mtak.hu/id/eprint/225158 |
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