Németh, Krisztina and Horváth, Flóra and Szatmári, Ágnes and Albitz, Evelin and Kele, Péter and Cserép, Balázs Gergely (2025) New Click on the Block: Photocaged Hydroxylamine, an Addition to the Light-Activatable Bioorthogonal Toolbox. CHEMISTRY-A EUROPEAN JOURNAL, 31 (42). No. e202501721. ISSN 0947-6539
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Abstract
A new photoactivatable bioorthogonal function was added to the photoactivatable bioorthogonal toolbox by transiently disabling the bioorthogonal reactivity of the hydroxylamine moiety with a photolabile protecting group. The photocaged bioorthogonal function was further equipped with a HaloTag substrate to enable its use in live-cell labeling schemes. Light-controlled recovery of reactivity was demonstrated by bioorthogonal fluorescent labeling of intracellular proteins in live cells.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund [NKFIH-K-143581]; The "Lenduelet" (Momentum) Program of Excellence of the Hungarian Academy of Sciences [LP-2024-11]; Hungarian Academy of Sciences [BO/00358/23/7] Funding text: This work has been implemented with the support provided by the Ministry of Innovation and Technology of Hungary from the National Research, Development and Innovation Fund (NKFIH-K-143581) and the "Lenduelet" (Momentum) Program of Excellence of the Hungarian Academy of Sciences (LP-2024-11). G.B.Cs. is grateful for the support of the Hungarian Academy of Sciences (BO/00358/23/7 Bolyai Research Fellowship). |
| Uncontrolled Keywords: | bioorthogonal; Light activation; photocage; caged hydroxylamine; |
| Subjects: | Q Science / természettudomány > QD Chemistry / kémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 25 Sep 2025 12:14 |
| Last Modified: | 25 Sep 2025 12:14 |
| URI: | https://real.mtak.hu/id/eprint/225370 |
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