Histone-like basic proteins on purified tobacco mosaic virus.

Balázs, Ervin and Tyihák, Ernő and Gáborjányi, Richárd (1977) Histone-like basic proteins on purified tobacco mosaic virus. CURRENT TOPICS IN PLANT BIOLOGY. pp. 333-337. ISSN 0972-4575

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Abstract

The U1 strain of tobacco mosaic virus (TMV) was shown to be intimately associated with basic proteins. The virus was purified by two cycles of differential centrifugation and the histone-like basic proteins were extracted from the purified virus with 0.25 N HC1 and Reinecke salt. After urea polyacrylamide gel electrophoresis, basic proteins separated as 6-7 bands which corresponded approximately to the position of calf thymus histones. The presence of histonelike basic proteins on the purified tobacco mosaic virus were also demonstrated on cellulose acetate membrane. Such basic protein may play an important role in virus replication but the location of the histones, their origin and their role in virus-host interaction remain to be determined.

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