Splicing of AN Intervening Sequence From Hybrid Cauliflower Mosaic Viral-rna

Hohn, Barbara and Balázs, Ervin and Ruegg, Doris and Hohn, Thomas (1986) Splicing of AN Intervening Sequence From Hybrid Cauliflower Mosaic Viral-rna. EMBO JOURNAL, 5 (11). pp. 2759-2762. ISSN 0261-4189

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Abstract

A DNA sequence encompassing intervening sequence 2 of the soybean leghemoglobin gene was inserted intő the cauliflower mosaic vírus (CaMV) genome. Upon passage through hőst plants progeny vírus slowly accumulated that had lost the intron, apparently by exact splicing of the RNA form of the genome at the exon/intron borders. These findings suggest that reverse transcription from (spliced) RNA to DNA is a normál step in CaMV replication. SÍ nuclease mapping revealed a mixed population of unspliced and spliced viral RNAs. The inefficiency of the splicing process is discussed.

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