Kovács, L. and Megyeri, Klára and Juhász, Anna and Zaja, Anikó and Miczák, A. (2004) Cloning, expression and purification OF SmpB from Mycobacterium tuberculosis. Acta Microbiologica et Immunologica Hungarica, 51 (3). pp. 297-302. ISSN 1217-8950
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Official URL: https://doi.org/10.1556/AMicr.51.2004.3.7
Abstract
SmpB, a small tmRNA binding protein, is essential for trans-translation. 6His and FLAG tagged SmpB was cloned from Mycobacterium tuberculosis H37Rv. It was expressed in Escherichia coli using the T7 promoter-polymerase system. Anti-FLAG M2 agarose was used for its purification. Mycobacterial SmpB copurifies with other proteins. We identified elongation factor EF-Tu in the purified SmpB preparations.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| Depositing User: | xFruzsina xPataki |
| Date Deposited: | 11 Sep 2017 18:48 |
| Last Modified: | 30 Nov 2024 00:15 |
| URI: | https://real.mtak.hu/id/eprint/62251 |
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