Leaf Polyphenols as Indicators of Climatic Adaptation of Beech (Fagus sylvatica L.) – an HPLC-MS/MS via MRM Approach

Hofmann, Tamás and Tálos-Nebehaj, Esztella and Albert, Levente (2017) Leaf Polyphenols as Indicators of Climatic Adaptation of Beech (Fagus sylvatica L.) – an HPLC-MS/MS via MRM Approach. International Labmate, 42 (3). pp. 12-14. ISSN 0143-5140

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Abstract

Temperate zone forests are heavily affected by climate change. Certain species like beech (Fagus sylvatica L.), common across Europe with high economic and ecological value are especially vulnerable and threatened by a retreat or possible extinction. A future task for forestry is to fi nd varieties which can adapt to changing climate due to their effi cient chemical defence mechanisms. Antioxidant leaf polyphenols represent one of these defence mechanisms. This work studied the relative quantitative evaluation of 44 compounds by HPLC-MS/MS via MRM peak areas as well as the determination of the antioxidant capacities from leaves of different beech varieties. Correlation analysis between parameters revealed that some of the most effi cient antioxidant polyphenols ((+)-Catechin, Procyanidin C trimer 3, Procyanidin B dimer 4) were found in the highest levels in the leaves of the most vulnerable varieties, with the poorest growing results and also with the highest antioxidant capacities. Results showed the novel potentials of HPLC-MS/MS in the research of biomarkers of climatic adapation in forest trees.

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