Analysis of oxylipins in human plasma: Comparison ofultrahigh-performance liquid chromatography andultrahigh-performance supercritical fluid chromatography coupled tomass spectrometry

Berkecz, Róbert and Lisa, Miroslav and Michal, Holˇcapek (2017) Analysis of oxylipins in human plasma: Comparison ofultrahigh-performance liquid chromatography andultrahigh-performance supercritical fluid chromatography coupled tomass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1511. pp. 107-121. ISSN 0021-9673

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Abstract

tThe potential of ultrahigh-performance liquid chromatography–mass spectrometry (UHPLC/MS) andultrahigh-performance supercritical fluid chromatography (UHPSFC) coupled to negative-ion electro-spray ionization mass spectrometry (ESI–MS) for the analysis of 46 oxylipins and 2 fatty acid standardsis compared in terms of their chromatographic resolution with the emphasis on distinguishing isobaricinterferences and the method sensitivity. UHPLC provides the baseline separation of 24 isobaric oxylipinswithin 13 min, while UHPSFC enables the separation of only 20 isobaric oxylipins within 8 min. More-over, the UHPLC/ESI–MS method provides an average improvement of sensitivity by 3.5-fold. A similartrend is observed in the analysis of human plasma samples, but lower ion suppression effects causedby lysophospholipids (LPL) are observed in case of UHPSFC/ESI–MS due to better separation of LPL. Bothmethods are fully applicable for the analysis of oxylipins, but UHPLC/ESI–MS method is preferred dueto better separation and higher sensitivity, which results in the identification of 31 oxylipins in humanplasma based on available standards and additional tentative 20 identifications based on accurate m/zvalues and the fragmentation behavior known from the literature.

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