N,N-Dimethylglycine Sodium Salt Exerts Marked Anti-Inflammatory Effects in Various Dermatitis Models and Activates Human Epidermal Keratinocytes by Increasing Proliferation, Migration and Growth Factor Release

Lendvai, Alexandra and Béke, Gabriella and Hollósi, Erika and Becker, Maike and Völker, Jörn Michael and Schulze zur Wiesche, Erik and Bácsi, Attila and Bíró, Tamás and Mihály, Johanna (2023) N,N-Dimethylglycine Sodium Salt Exerts Marked Anti-Inflammatory Effects in Various Dermatitis Models and Activates Human Epidermal Keratinocytes by Increasing Proliferation, Migration and Growth Factor Release. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (24). No.-11264. ISSN 1661-6596

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Abstract

N,N-Dimethylglycine (DMG) is a naturally occurring compound being widely used as an oral supplement to improve growth and physical performance. Thus far, its effects on human skin have not been described in the literature. For the first time, we show that N,N-Dimethylglycine sodium salt (DMG-Na) promoted the proliferation of cultured human epidermal HaCaT keratinocytes. Even at high doses, DMG-Na did not compromise cellular viability of these cells. In a scratch wound closure assay, DMG-Na augmented the rate of wound closure, demonstrating that it promotes keratinocyte migration. Further, DMG-Na treatment of the cells resulted in the upregulation of the synthesis and release of specific growth factors. Intriguingly, DMG-Na also exerted robust anti-inflammatory and anti-oxidant effects as assessed in three different models of human keratinocytes, mimicking microbial and allergic contact dermatitis as well as psoriasis and UVB irradiation induced solar dermatitis. These results identify DMG-Na as a highly prom-ising novel active to promote epidermal proliferation, regeneration and repair, and to exert protective functions. Further pre-clinical and clinical studies are under investigation to prove the seminal impact of topically applied DMG-Na on relevant conditions of the skin and its append-ages.

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