Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components ofN-Glycoproteins

Ágoston, Károly and Gyémánt, Gyöngyi and Kalmár, László and Kerékgyártó, János and Szurmai, Zoltán and Döncző, Boglárka and Guttman, András (2014) Synthesis and MALDI-TOF MS Analysis of Protected Oligosaccharide Components ofN-Glycoproteins. Journal of Carbohydrate Chemistry, 33 (6). pp. 326-343. ISSN 0732-8303

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Abstract

Essential components of N-glycoproteins were synthesized in octyl glycoside form starting from 3-O-allyl-D-glucose derivative. The β-mannosidic linkage was formed by the oxidation reduction method. MALDI-TOF mass spectrometry and its post-source decay (PSD) mode were used for identification and structural elucidation of protected synthetic oligosaccharides related to N-glycoproteins. Most fragments, identified in the PSD spectra, were products of the cleavage of glycosidic bonds.

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