REAL

Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry

Hofmann, Tamás and Nebehaj, Esztella and Albert, Levente (2016) Antioxidant properties and detailed polyphenol profiling of European hornbeam (Carpinus betulus L.) leaves by multiple antioxidant capacity assays and high-performance liquid chromatography/multistage electrospray mass spectrometry. Industrial Crops and Products, 87. pp. 340-349. ISSN 0926-6690

[img] Text
2016_Hofmann_et_al_ICP_Carpinus_betulus_leaf_LCMS_AOK.pdf
Restricted to Registered users only

Download (822kB)

Abstract

According to recent studies, the antioxidant and anticancer related properties of European hornbeam (Carpinus betulus L.) leaf extracts are remarkable, making it a possible raw material for medicine, requiring the identification of major active compounds and the assessment of the antioxidant properties of the extracts. In the present work the high-performance liquid chromatographic/multistage mass spectrometric characterization of the antioxidant polyphenolic compounds as well as the evaluation of the seasonal changes in the major antioxidant capacity and related parameters (DPPH, ABTS, FRAP, total phenol content, total flavonoid content and total flavan-3-ol content) have been carried out for the leaves of European hornbeam for the first time. The extracts exhibited the highest antioxidant capacity in August, basing on the FRAP (106.24 ± 3.10 mg AAE/g dw.) and DPPH (IC50: 4.63 ± 0.88 μg/ml) assays and in May, based on the ABTS (329.78 ± 23.89 mg TE/g dw.) method. From the August extract a total of 171 compounds, including phenolic acids, flavonoid glycosides, tannins, catechins and procyanidins have been characterized and identified. According to the HPLC-PDA peak areas, the most abundant polyphenolic compounds in the August extracts were chlorogenic acid, ellagic acid, ellagitannins, myricetin-, luteolin-, quercetin- and apigenin glycosides. Further research is needed for the elucidation of the compounds which are primarily responsible for the antioxidant properties. © 2016 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: Chromatography; Flavonoids; High performance liquid chromatography; Liquid chromatography; Liquids; Mass spectrometry; Phenols; Polyphenolic compounds; Spectrometry; Sugars, Antioxidant capacity; Antioxidant properties; Carpinus; Electrospray mass spectrometry; European hornbeams; Polyphenols; Total flavonoid contents; Total phenol contents, Antioxidants, Carpinus betulus
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
S Agriculture / mezőgazdaság > SD Forestry / erdőgazdaság
Depositing User: Dr Tamás Hofmann
Date Deposited: 21 Sep 2016 12:54
Last Modified: 21 Sep 2016 12:54
URI: http://real.mtak.hu/id/eprint/39774

Actions (login required)

Edit Item Edit Item