Separation and identification of antibacterial chamomile components using OPLC, bioautography and GC-MS

Móricz, Ágnes M. and Szarka, Szabolcs and Ott, Péter G. and Héthelyi, Éva B. and Szőke, Éva and Tyihák, Ernő (2012) Separation and identification of antibacterial chamomile components using OPLC, bioautography and GC-MS. Medicinal Chemistry, 8 (1). pp. 85-94.

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Abstract

Components of 50% ethanolic chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line OPLC, which consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected peaks were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% ethanolic extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone.

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