Assessment of biofilm formation of Listeria monocytogenes strains

Mráz, B. and Kiskó, G. and Hidi, E. and Ágoston, R. and Mohácsi-Farkas, Cs. and Gillay, Z. (2011) Assessment of biofilm formation of Listeria monocytogenes strains. Acta Alimentaria, 40 (Supple). pp. 101-108. ISSN 0139-3006

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Abstract

Biofilm formation of four Listeria monocytogenes strains with different origin was compared as a function of surface material (stainless steel, polystyrene and glass) and surface conditioning. Hydrophobicity of strains was also examined. The number of adhered cells in PBS broth was determined for all strains during 144 h of incubation at 30 °C with epifluorescent microscopy. L. monocytogenes strains were similar in biofilm forming abilities. The slight differences in biofilm formation could not been explained by hydrophobicity. Results also showed that stainless steel provided the best surface for biofilm formation. The effect of pre-conditioning of the surfaces with milk was dependent on the surface material. Significantly lower attachment could be observed on milk conditioned surfaces in case of cheese isolate.

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