Overpressured layer chromatography: from the pressurized ultramicro chamber to BioArena system

Tyihák, Ernő and Mincsovics, Emil and Móricz, Ágnes M. (2012) Overpressured layer chromatography: from the pressurized ultramicro chamber to BioArena system. Journal of Chromatography A, 1232. pp. 3-18.

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Abstract

The pressurized ultramicro (UM) chamber as a closed adsorbent layer chamber enables the use of a special chromatoplate and a pump to increase and optimize the mobile phase flow velocity through an optional development distance in an adsorbent layer. This chamber is the basic instrument of overpressured-layer chromatography (OPLC), which is a separation technique that combines the advantages of conventional TLC/HPTLC with those of HPLC. The versions of OPLC instrument, the character and achievement of off-line and on-line OPLC systems in analytical and preparative use are described. The development of BioArena as a complex bioautographic system means an exploitation of the unique advantages of planar-layer system for detection, isolation and identification of new antimicrobials, antineoplastics, biopesticides and other biologically active substances as well as for studying fundamental biochemical reactions and mechanisms.

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