Olajos, Gábor and Bartus, Éva and Schuster, Ildikó and Lautner, Gergely and Gyurcsányi, Ervin Róbert and Szögi, Titanilla and Fülöp, Lívia and Martinek, Tamás (2017) Multivalent foldamer-based affinity assay for selective recognition of Aβ oligomers. Analytica Chimica Acta. pp. 1-18. ISSN 0003-2670
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Abstract
Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10–500 pM. These results suggest that protein mimetic foldamers can be useful tools in biosensors and affinity assays.
Item Type: | Article |
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Subjects: | Q Science / természettudomány > Q1 Science (General) / természettudomány általában Q Science / természettudomány > QD Chemistry / kémia Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
Depositing User: | Berényi Szilvia |
Date Deposited: | 07 Feb 2017 15:22 |
Last Modified: | 04 Apr 2023 12:15 |
URI: | http://real.mtak.hu/id/eprint/47581 |
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